Rasusa¶
Introduction¶
Rasusa: Randomly subsample sequencing reads to a specified coverage.
For more information, please check:
Docker hub: https://hub.docker.com/r/staphb/rasusa
Home page: https://github.com/mbhall88/rasusa
Versions¶
0.6.0
0.7.0
Commands¶
rasusa
Module¶
You can load the modules by:
module load biocontainers
module load rasusa
Example job¶
Warning
Using #!/bin/sh -l
as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash
instead.
To run rasusa on our clusters:
#!/bin/bash
#SBATCH -A myallocation # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=rasusa
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out
module --force purge
ml biocontainers rasusa
rasusa -i seq_1.fq -i seq_2.fq \
--coverage 100 --genome-size 35mb \
-o out.r1.fq -o out.r2.fq