relion

Description

RELION for REgularized LIkelihood OptimizatioN implements an empirical Bayesian approach for analysis of electron cryo-microscopy Cryo-EM. Specifically it provides methods of refinement of singular or multiple 3D reconstructions as well as 2D class averages. RELION is an important tool in the study of living cells.

Versions

  • Scholar: 2.1.b1, 3.0.8, 3.1.0, 3.1.2, 3.1.3

  • Gilbreth: 2.1.b1, 3.0.8, 3.1.0, 3.1.2, 3.1.3

  • Anvil: 2.1.b1, 3.1.0, 3.1.2, 3.1.3

Module

You can load the modules by:

module load ngc
module load relion

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run relion on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH -c 8
#SBATCH --gpus-per-node=1
#SBATCH --job-name=relion
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml ngc relion